绵羊LPIN1基因的克隆和mRNA表达研究

doi:10.11843/j.issn.0366-6964.2013.09.006

Abstract

Abstract:

This study aimed to clone ovine LPIN1 gene and to analyze its ontogenetic mRNA expression in seven adipose tissues of two breeds with distinct tail types, and based on which, to reveal the role of the gene in fat metabolism of sheep. RT-PCR technique was used to amplify LPIN1 gene. Bioinformatics methods were used to predict the physicochemical properties and domains in Lipin1 protein. Ninety six animals from Guangling Large Tailed and Small Tailed Han (48 for each breed with equal sex ratios) were slaughtered at 2, 4, 6, 8, 10 and 12 months of age, respectively. Adipose tissues were collected from great and small omental, mesenteric, retroperitoneal, subcutaneous, perirenal and tail fats to study the ontogenetic mRNA expression by real-time PCR. The results showed that the coding region was 2 688 bp in length, coding 895 amino acids. Lipin1 was a kind of unstable and hydrophilic protein with 10 potential glycosylation and 88 phosphorylation sites, but without transmembrane domain and signal peptide. N-and C-terminals contained two highly conserved regions(Lipin-N and LNS2 (Lipin/Ned1/Smp2)). LPIN1 mRNA expressed in all adipose tissues studied, but the highest in perirenal fat and the lowest in mesenteric fats. Months of age, tissue and breed as well as their twoway interactions had significant influences on the LPIN1 mRNA expression. LPIN1 gene is significant in the genetic improvement of meat quality traits of sheep based on its direct participating in and indirect regulating fat metabolism.

CLC Number:

S826
S813.3

WEI Lin-lin, GAO Jin-sheng, WANG Jing-lin, CHENG Li-fen, QIAO Li-ying, JIA Xia-li, ZHANG Jing, YANG Kai-jie, LIU Jian-hua, LIANG Chen, LIU Wen-zhong. Study on the Cloning and Ontogenetic mRNA Expression of Ovine LPIN1 Gene[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2013, 44(9): 1371-1379.

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References

［1］PÉTERFY M, PHAN J, XU P, et al. Lipodystrophy in the fld mouse results from mutation of a new gene encoding a nuclear protein, lipin［J］. Nat Genet, 2001, 27(1): 121-124.

［2］HAN G S, WU W I, CARMAN G M. The Saccharomyces cerevisiae Lipin homolog is a Mg²⁺- dependent phosphatidate phosphatase enzyme［J］. Biol Chem, 2006, 281(14): 9210-9218.

［3］CARMAN G M, HAN G S. Phosphatidic acid phosphatase, a key enzyme in the regulation of lipid synthesis［J］. Biol Chem, 2009, 284(5): 2593-2597.

［4］REUE K, BRINDLEY D N. Multiple roles for lipins/phosphatidate phosphatase enzymes in lipid metabolism［J］. Lipid Res, 2008, 49(12): 2493-2503.

［5］FINCK B N, GROPLER M C, CHEN Z, et al. Lipin 1 is an inducible amplifier of the hepatic PGC- 1alpha/PPARalpha regulatory pathway［J］. Cell Metab, 2006,4(3): 199-210.

［6］PÉTERFY M, PHAN J, REUE K. Alternatively spliced lipin isoforms exhibit distinct expression pattern, subcellular localization, and role in adipogenesis［J］. Biol Chem, 2005, 280(38): 32883-32889.

［7］PHAN J, REUE K. Lipin, a lipodystrophy and obesity gene［J］. Cell Metab, 2005, 1(1): 73-83.

［8］YAO-BORENGASSER A, RASOULI N, VARMA V, et al. Lipin expression is attenuated in adipose tissue of insulin-resistant human subjects and increases with peroxisome proliferator-activated receptor gamma activation［J］. Diabetes, 2006, 55(10): 2811-2818.

［9］HE X P, XU X W, ZHAO S H, et al. Investigation of Lpin1 as a candidate gene for fat deposition in pigs［J］. Mol Biol Rep, 2009, 36(5): 1175-1180.

［10］HE X, XU X, LIU B. Molecular characterization, chromosomal localization and association analysis with back-fat thickness of porcine LPIN2 and LPIN3 ［J］. Mol Biol Rep, 2009,36(7):1819-1824.

［11］卫利选，常振华，何诚，等. LPIN1基因多态性与黄牛经济性状的关联研究［J］. 西北农业学报, 2012, 21(1): 11-15.

［12］RUDOLPH M C, MCMANAMAN J L, PHANG T, et al. Metabolic regulation in the lactating mammary gland: a lipid synthesizing machine［J］. Physiol Genomics, 2007, 28(3): 323-336.

［13］GONZÁLEZ C R, NOVELLE M G, CAMINOS J E, et al. Regulation of lipin1 by nutritional status, adiponectin, sex and pituitary function in rat white adipose tissue［J］. Physiol Behav, 2012, 105(3): 777-783.

［14］SUVIOLAHTI E, REUE K, CANTOR R M, et al. Cross-species analyses implicate Lipin 1 involvement in human glucose metabolism［J］. Hum Mol Genet, 2006, 15(3): 377-386.

［15］WANG Q, JI C, HUANG J, et al. The mRNA of lipin1 and its isoforms are differently expressed in the longissimus dorsi muscle of obese and lean pigs［J］. Mol Biol Rep, 2011, 38(1): 319-325.

［16］WANG X K, CHEN W, HUANG Y Q, et al. Identification of the transcript isoforms and expression characteristics for chicken Lpin1［J］. Animal, 2012, 6(12): 1897-1903.

［17］DONKOR J, SARIAHMETOGLU M, DEWALD J, et al. Three mammalian lipins act as phosphatidate phosphatases with distinct tissue expression patterns［J］. Biol Chem, 2007, 282(6): 3450-3457.

［18］杨静, 李成云, 王云月, 等. 酿酒酵母分泌蛋白组的计算机分析［J］. 中国农业科学, 2005, 38(3): 516-522.

［19］OHTSUBO K, MARTH J D. Glycosylation in cellular mechanisms of health and disease［J］. Cell, 2006, 126(5): 855-867.

［20］CIES′LA J, FRACZYK T, RODE W. Phosphorylation of basic amino acid residues in proteins: important but easily missed［J］. Acta Biochimica Polonica, 2011, 58: 137-148.

［21］HUFFMAN T A, MOTHE-SATNEY I, LAWRENCE JC J R. Insulin-stimulated phosphorylation of lipin mediated by the mammalian target of rapamycin［J］. Proc Natl Acad Sci USA, 2002, 99(2): 1047-1052.

［22］REUE K, ZHANG P. The lipin protein family: dual roles in lipid biosynthesis and gene expression［J］. FEBS Lett, 2008, 582(1): 90-96.

［23］林婄婄, 高中元, 袁亚男, 等. PPARα和PPARγ在不同脂尾型绵羊脂肪组织中的发育性表达研究［J］. 畜牧兽医学报, 2012, 43(9): 1369-1376.

［24］ZHANG P, TAKEUCHI K, CSAKI L S, et al. Lipin-1 phosphatidic phosphatase activity modulates phosphatidate levels to promote peroxisome proliferator-activated receptor γ (PPARγ) gene expression during adipogenesis［J］. Biol Chem, 2012, 287(5): 3485-3494.

［25］REUE K, DWYER J R. Lipin proteins and metabolic homeostasis［J］. Lipid Res, 2009, 50(Suppl): S109-114.

［26］ZHANG P, OLOUGHLIN L, BRINDLEY D N, et al. Regulation of lipin-1 gene expression by glucocorticoids during adipogenesis［J］. Lipid Res, 2008, 49(7): 1519-1528.

［27］LI S, CHEN W, KANG X, et al. Distinct tissue expression profiles of chicken Lpin1-α/β isoforms and the effect of the variation on muscle fiber traits［J］. Gene, 2013, 515(2): 281-290.

［28］YUAN Y N, LIU W Z, LIU J H, et al. Cloning and ontogenetic expression of the uncoupling protein 1 gene UCP1 in sheep［J］. Appl Genet, 2012, 53(2): 203-212.

［29］LU B, LUY, MOSER A H, et al. LPS and proinflammatory cytokines decrease lipin-1 in mouse adipose tissue and 3T3-L1 adipocytes［J］. Am J Physiol Endocrinol Metab, 2008, 295(6): E1502-1509.

［30］TSUCHIYA Y, TAKAHASHI N, YOSHIZAKI T, et al. A Jak2 inhibitor, AG490, reverses lipin-1 suppression by TNF-alpha in 3T3-L1 adipocytes［J］. Biochem Biophys Res Commun, 2009, 382(2): 348-352.

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Study on the Cloning and Ontogenetic mRNA Expression of Ovine LPIN1 Gene

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